{"id":750,"date":"2022-05-13T11:26:45","date_gmt":"2022-05-13T11:26:45","guid":{"rendered":"https:\/\/hybrid-course.i3s.up.pt\/?page_id=750"},"modified":"2022-07-19T14:01:45","modified_gmt":"2022-07-19T14:01:45","slug":"materials-labwork","status":"publish","type":"page","link":"https:\/\/hybrid-course.i3s.up.pt\/?page_id=750","title":{"rendered":"Materials: Labwork"},"content":{"rendered":"\n
<\/div>
\n
\n

COURSE MATERIALS <\/span><\/strong><\/p><\/div><\/div>\n\n\n\n

Unboxing CRISPR<\/span><\/h2><\/div>

for transdisciplinary learning
<\/span><\/h4><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n

Materials: <\/strong>Labwork <\/strong><\/h1>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
\n
\n

Introduction to gene edition – hands-on<\/a><\/h2>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
<\/i><\/div>

Inside a CRISPR Lab<\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div>
(6\u201937\u2019\u2019)Video on the work of researchers at UC Berkeley using CRISPR. The video allows you to take a peek behind the scenes to see what goes into a human genome editing experiment and follow the process <\/div><\/div>
MORE<\/i><\/a><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n
\n

DNA and RNA degradation<\/a><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
<\/i><\/div>

General precautions when handling RNA  <\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div>
A Text with illustration explaining:  a) general concepts and function of RNA, DNA, RNAase; degradation of RNA; b)  the major sources of RNase contamination in a typical laboratory; c) precautions to avoid RNase contamination.<\/div><\/div>
PDF<\/i><\/a><\/div><\/div><\/div><\/div><\/div>\n\n\n\n

<\/p>\n<\/div><\/div><\/div>\n\n\n\n

\n

Cutting DNA<\/a><\/h2>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
<\/i><\/div>

Cutting DNA<\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div>
This is a basic protocol to cut DNA into smaller fragments. The cut is nonspecific, being an excellent example of contrast with the specificity of CRISPR\/cas9.<\/div><\/div>
PDF<\/i><\/a><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n
\n

Electrophoresis<\/a><\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
<\/i><\/div>

Electrophoresis<\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div>
This is a protocol to do an agarose gel electrophoresis, a standard lab procedure used to separate macromolecules based on size. The technique applies a negative charge so proteins move towards a positive charge. Electrophoresis is used extensively in DNA, RNA, and protein analysis.<\/div><\/div>
PDF<\/i><\/a><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n
\n
\n

In Silico<\/em> sgRNA design<\/a><\/h2>
<\/span><\/div><\/div><\/div><\/div>\n\n\n\n
<\/i><\/div>

In silico lab work: CRISPR cas9 gene editing, F\u00e1bio Ferreira<\/strong><\/strong><\/strong><\/h2>
<\/span><\/div><\/div><\/div>
(28\u201947\u2019\u2019) F\u00e1bio J\u00fanio Ferreira, i3S researcher, introduces how to design a sgRNA, particularly to edit Streptomycetes and zebrafish. <\/div><\/div>
MORE<\/i><\/a><\/div><\/div><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n
\n