{"id":638,"date":"2022-05-12T10:23:14","date_gmt":"2022-05-12T10:23:14","guid":{"rendered":"https:\/\/hybrid-course.i3s.up.pt\/?page_id=638"},"modified":"2022-05-23T13:13:35","modified_gmt":"2022-05-23T13:13:35","slug":"structure","status":"publish","type":"page","link":"https:\/\/hybrid-course.i3s.up.pt\/?page_id=638","title":{"rendered":"Structure"},"content":{"rendered":"\n<div class=\"wp-block-qubely-row alignfull qubely-section qubely-block-4d564e\"><div class=\"qubely-row-overlay\"><\/div><div class=\"qubely-container\"><div class=\"qubely-row \">\n<div class=\"wp-block-qubely-column qubely-column qubely-column-front qubely-block-83d112\"><div class=\"qubely-column-inner\">\n<div class=\"wp-block-qubely-text qubely-block-214369\"><div class=\"qubely-block-text  \"><p><strong><span style=\"color: var(--theme-palette-color-8, #ffffff);\" class=\"stk-highlight\">COURSE STRUCTURE<\/span><\/strong><\/p><\/div><\/div>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-fcd6db\" id=\"2--let%E2%80%99s-change-how-we-manage-business-\"><div class=\"qubely-block-heading  \"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\"><span style=\"color: var(--theme-palette-color-8, #ffffff);\" class=\"stk-highlight\"><span style=\"color: var(--theme-palette-color-8, #ffffff);\" class=\"stk-highlight\">Unboxing CRISPR <\/span><\/span><\/h2><\/div><h4 class=\"qubely-sub-heading-selector\"><span style=\"color: var(--theme-palette-color-8, #ffffff);\" class=\"stk-highlight\">for transdisciplinary learning<\/span><\/h4><\/div><\/div>\n<\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-50646c\"><div class=\"qubely-block-heading  qubely-has-separator qubely-separator-position-bottom\"><div class=\"qubely-heading-container\"><h1 class=\"qubely-heading-selector\">Structure<\/h1><div class=\"qubely-separator qubely-separator-after\"><span class=\"qubely-separator-type-css qubely-separator-solid\"><\/span><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-columns stk-block-columns stk-block stk-23e9961\" data-block-id=\"23e9961\"><style>.stk-23e9961{margin-bottom:40px !important}<\/style><div class=\"stk-row stk-inner-blocks stk-block-content stk-content-align stk-23e9961-column\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-e9be460\" data-block-id=\"e9be460\"><style>.stk-e9be460-container{margin-top:25px !important;margin-right:30px !important;margin-left:0px !important}<\/style><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-e9be460-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks\">\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-3230bdc\" data-block-id=\"3230bdc\"><style>.stk-3230bdc{height:70px !important}<\/style><\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"576\" src=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-1024x576.jpg\" alt=\"\" class=\"wp-image-643\" srcset=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-1024x576.jpg 1024w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-300x169.jpg 300w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-768x432.jpg 768w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-1536x864.jpg 1536w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF2835-2048x1152.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-9f5ea07\" data-block-id=\"9f5ea07\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-9f5ea07-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks\">\n<div class=\"wp-block-qubely-row qubely-section qubely-block-201d10\"><div class=\"qubely-row-overlay\"><\/div><div class=\"qubely-container\"><div class=\"qubely-row \">\n<div class=\"wp-block-qubely-column qubely-column qubely-column-front qubely-block-3c8bbf\"><div class=\"qubely-column-inner\">\n<div class=\"wp-block-qubely-heading qubely-block-906c12\" id=\"3--about-the-course-and-requirements-%C2%A0\"><div class=\"qubely-block-heading  qubely-has-separator qubely-separator-position-bottom\"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\"><strong>Course Structure<\/strong><\/h2><div class=\"qubely-separator qubely-separator-after\"><span class=\"qubely-separator-type-css qubely-separator-solid\"><\/span><\/div><\/div><\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">This pilot course was designed to allow you to adapt it to your context (country, education level, field of study) and so doesn\u2019t have a rigid structure. Instead, we propose a series of materials and a general organization of exercises and topics interweaving theoretical and practical, scientific and artistic contents and approaches.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">However, you can easily adapt the proposed structure and contents to best fit your needs and circumstances.<\/p>\n<\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-columns stk-block-columns stk-block stk-ce5d89d\" data-block-id=\"ce5d89d\"><style>.stk-ce5d89d{margin-bottom:60px !important}<\/style><div class=\"stk-row stk-inner-blocks stk-block-content stk-content-align stk-ce5d89d-column\">\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-7cf9381\" data-block-id=\"7cf9381\"><style>.stk-7cf9381-container{margin-top:25px !important;margin-right:30px !important;margin-left:0px !important}<\/style><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-7cf9381-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks\">\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-9d7e50a\" data-block-id=\"9d7e50a\"><style>.stk-9d7e50a{height:50px !important}<\/style><\/div>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-1ff31d\" id=\"3--about-the-course-and-requirements-%C2%A0\"><div class=\"qubely-block-heading  qubely-has-separator qubely-separator-position-bottom\"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\"><strong>General Structure (proposal) <\/strong><\/h2><div class=\"qubely-separator qubely-separator-after\"><span class=\"qubely-separator-type-css qubely-separator-solid\"><\/span><\/div><\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-e950a91\" data-block-id=\"e950a91\"><style>.stk-e950a91{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">What\u2019s CRISP-R? (general explanation and debate)&nbsp; | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-50dd969\" data-block-id=\"50dd969\"><style>.stk-50dd969{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Arts assignments or creative prompts to promote creativity | 2h | PRACTICAL<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-03bd6b7\" data-block-id=\"03bd6b7\"><style>.stk-03bd6b7{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Exploring the art\/science relationship and the concept of bio-art  | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-7e8837c\" data-block-id=\"7e8837c\"><style>.stk-7e8837c{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Basic concepts of molecular biology: DNA, protein, genes, RNA | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-d2d76bb\" data-block-id=\"d2d76bb\"><style>.stk-d2d76bb{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Introduction to gene edition &#8211; hands-on &amp;  DNA \/ RNA degradation  | 2h | LABWORK<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-0e35f52\" data-block-id=\"0e35f52\"><style>.stk-0e35f52{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Cutting DNA &amp;  Gel Electrophoresis | 4h | LABWORK<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-af30d45\" data-block-id=\"af30d45\"><style>.stk-af30d45{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Introduction to Gene Editing | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-14fb53f\" data-block-id=\"14fb53f\"><style>.stk-14fb53f{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Introducing artworks exploring gene editing | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-0905c5d\" data-block-id=\"0905c5d\"><style>.stk-0905c5d{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">CRISP-R: how does it work | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-c094c84\" data-block-id=\"c094c84\"><style>.stk-c094c84{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Design &amp; artworks exploring CRISPR | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-119e2fc\" data-block-id=\"119e2fc\"><style>.stk-119e2fc{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Group Project: problem-solving with CRISPR | 4h | PRACTICAL  <br>Design a project to address a problem in today\u2019s world using CRISPR <\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-a281d76\" data-block-id=\"a281d76\"><style>.stk-a281d76{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">In Silico CRISP-R (sgRNA) | 2h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-218a4bb\" data-block-id=\"218a4bb\"><style>.stk-218a4bb{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">In Silico sgRNA design  | 2h | LABWORK <br>Speculative imaginations by artists | 1h | THEORY<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-27dff8f\" data-block-id=\"27dff8f\"><style>.stk-27dff8f{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Playing with the genetic code | 3h | PRACTICAL<br>Design your sgRNA exploration (for the group project)<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-3197980\" data-block-id=\"3197980\"><style>.stk-3197980{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">In vitro gene editing &#8211; sgRNA synthesis, RNA purification, Gel Electrophoresis, run, and analysis | 4h | THEORY + LABWORK<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-a9761b2\" data-block-id=\"a9761b2\"><style>.stk-a9761b2{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">In vitro cleavage | 4h | THEORY + LABWORK<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-74ff319\" data-block-id=\"74ff319\"><style>.stk-74ff319{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Problem-solving with CRISP-R  | 1h | PRACTICAL<br>Methodology: What Lab work do I need to do? <\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-9c7855a\" data-block-id=\"9c7855a\"><style>.stk-9c7855a{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">CONSULTING WITH SCIENTISTS | 3h | PRACTICAL<br>Group work with researchers discussing the possibility of making the projects possible and on the limitations of science <\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-a08c7db\" data-block-id=\"a08c7db\"><style>.stk-a08c7db{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Adjusting the projects &amp; project development | 4h | PRACTICAL<\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-366fd19\" data-block-id=\"366fd19\"><style>.stk-366fd19{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Presentation of project prototypes | 3h |  PRACTICAL <\/p><\/div>\n\n\n\n<div class=\"wp-block-stackable-text stk-block-text stk-block stk-80ef00b\" data-block-id=\"80ef00b\"><style>.stk-80ef00b{margin-bottom:0px !important}<\/style><p class=\"stk-block-text__text\">Reflection on the CRISP-R powers and limitations | 1h | PRACTICAL   <\/p><\/div>\n\n\n\n<div style=\"height:82px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-qubely-heading qubely-block-1e71eb\" id=\"3--about-the-course-and-requirements-%C2%A0\"><div class=\"qubely-block-heading  qubely-has-separator qubely-separator-position-bottom\"><div class=\"qubely-heading-container\"><h2 class=\"qubely-heading-selector\"><strong><strong>Didactic exploration proposal<\/strong>&nbsp;<\/strong><\/h2><div class=\"qubely-separator qubely-separator-after\"><span class=\"qubely-separator-type-css qubely-separator-solid\"><\/span><\/div><\/div><\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\">This STEAM module for higher education is a multi-disciplinary approach focused on the subject matter of art &amp; science, knowledge, and hands-on experiences, to address connections between technological and biological realms. It aims to equip the students with the ability to understand new concepts in different STEAM subjects, think (and experiment) critically and creatively, and act to develop and apply innovative ideas. In that sense, it also aims to integrate STEAM content with practical activities that promote the development of several skills ranging from creative thinking, laboratory skills, and learning molecular biology techniques and scientific and artistic thinking skills. Other skills are involved such as student collaboration and reflection in a learning-through-design project as well as communication and peer persuasion. Unboxing CRISPR can be structured to be taught over 13 steps. Below we describe the structure of those 13 steps by briefly outlining the topics and teaching and learning activities that can take place in each step.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>1<sup>st<\/sup> step<\/strong> \u2013 Students begin by exploring <em>What&#8217;s CRISPR and how it can impact life, during a theoretical class. <\/em>Afterward, in a practical class (2h), students will explore creative thinking techniques, using some creative prompts, to generate creative ideas on how to solve problems and change reality with CRISPR. The resulting ideas may then guide the group project to be developed during the course (step 6).&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>2<sup>nd<\/sup> and 3<sup>rd<\/sup> steps <\/strong>\u2013 can be centered on scientific, theoretical, and practical knowledge. Thus, in the 2nd step, two hours of theoretical classes should be dedicated to Basic concepts of molecular biology: DNA, protein, genes, and <em>RNA<\/em>. The learning outcome is to understand the basic knowledge, especially around the foundations of genetics and CRISPR technology. This should be followed by, at least, two hours of practical laboratory work related to &#8211; <em>Introduction to gene editing<\/em> &#8211; <em>hands-on and DNA \/ RNA degradation<\/em>.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>3<sup>rd<\/sup> step <\/strong>\u2013 should be dedicated <em>to General precautions when handling RNA. <\/em>Laboratory good practices to work with RNA need to be addressed so students learn about safety laboratory measures and how to avoid RNase contamination. Students should learn the fundamental skills of molecular biology techniques involving DNA, and RNA manipulation (4h), beginning with how <em>to cut <\/em>DNA and visualize DNA fragments using e<em>lectrophoresis.<\/em>&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>4<sup>th<\/sup> step &#8211; <\/strong>in this step the classes should focus on merging science and arts. A theoretical introduction to gene editing (theoretical class), should be followed by a session with an art expert to introduce artworks exploring gene editing. Students should discuss the topic of <em>how artists use art to explore this genome-editing technology.<\/em> These artworks serve as triggers for discussion and reflection on Art &amp; Science &amp; Technology, as well as inspiration for the group projects.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>5<sup>th<\/sup> step<\/strong> \u2013 should be dedicated to understanding CRISPR both from a scientific point of view and its\u2019 intersection with art and design. It consists of a theoretical exploration of CRISPR (2h of theoretical lecture), the presentation of design &amp; artworks exploring CRISPR (2h), and debate and reflection. The idea is to inspire students to design a project for problem-solving with CRISPR (Design\/Art project) (step 1). In that sense, a design thinking approach and problem-based learning can be adopted, for students to design, develop, and build their own projects.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>6<sup>th<\/sup> step<\/strong> \u2013 should be focused on collaborative work where students from diverse backgrounds design a project addressing the question &#8211; <em>What problem would you like to try to solve with CRISPR?<\/em> The answer to this question requires creative thinking to explore the relationships between CRISPR-Cas9 and Art and Design (Design \/ Art project) (step 2). This problem-exploration phase involves autonomous research, multidisciplinary reflection, and discussion, as well as the engagement and discussion with experts in diverse areas.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>7<sup>th<\/sup> step &#8211; <\/strong>should focus on <em>Virtual Gene Editing<\/em>, connecting theory with some practical examples, on theoretical-practical classes (2h) about <em>In Silico CRISPR (sgRNA)<\/em>. This activity addresses the concepts behind genomes, mutations, and CRISPR\/Cas edition. The session on <em>sgRNA in silico design <\/em>is an in silico lab work (2h) to design the guiding RNAs for the enzyme activity. Available online tools can be used to create single guide RNA and, therefore, target-specific genome regions with CRISPR, in distinct species.&nbsp;<\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-column stk-block-column stk-column stk-block stk-370fdf7\" data-block-id=\"370fdf7\"><div class=\"stk-column-wrapper stk-block-column__content stk-container stk-370fdf7-container stk--no-background stk--no-padding\"><div class=\"stk-block-content stk-inner-blocks\">\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-0bb1c75\" data-block-id=\"0bb1c75\"><style>.stk-0bb1c75{height:60px !important}<\/style><\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1923\" src=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-scaled.jpg\" alt=\"\" class=\"wp-image-647\" srcset=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-scaled.jpg 2560w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-300x225.jpg 300w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-1024x769.jpg 1024w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-768x577.jpg 768w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-1536x1154.jpg 1536w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/DSCF8411-edited-2048x1538.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"2560\" src=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-scaled.jpg\" alt=\"\" class=\"wp-image-659\" srcset=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-scaled.jpg 2560w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-300x300.jpg 300w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-1024x1024.jpg 1024w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-150x150.jpg 150w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-768x768.jpg 768w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-1536x1536.jpg 1536w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_1420-edited-2048x2048.jpg 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"2281\" height=\"2281\" src=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited.jpg\" alt=\"\" class=\"wp-image-660\" srcset=\"https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited.jpg 2281w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-300x300.jpg 300w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-1024x1024.jpg 1024w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-150x150.jpg 150w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-768x768.jpg 768w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-1536x1536.jpg 1536w, https:\/\/hybrid-course.i3s.up.pt\/wp-content\/uploads\/2022\/05\/IMG_3443-2-edited-2048x2048.jpg 2048w\" sizes=\"auto, (max-width: 2281px) 100vw, 2281px\" \/><\/figure>\n\n\n\n<div style=\"height:88px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>9<sup>th<\/sup> and 10<sup>th<\/sup> steps \u2013 <\/strong>these steps should involve the complete lab process of <em>In vitro gene editing<\/em>. It should start with a theoretical-practical class about an <em>Overview of the CRISPR\/Cas9 system<\/em> (1h) aiming to explain what is the CRISPR-Cas9 system. During the lab work activity (3h) students will put their hands on the protocol by synthesizing the sgRNA (1h40) &#8211; RNA transcription using T7 RNA polymerase using DNA as a template; Purification of RNA (10 min); RNA synthesis confirmation in agarose gel (1h15). The <em>Analysis of purified sgRNA by gel electrophoresis<\/em> objective is to run the synthesized purified product in a gel to see if the purification worked. But it also allows a rough quantification of the sgRNA, based on the intensity of fluorescence of the bands.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>10<sup>th<\/sup> <\/strong>step<strong> &#8211; <\/strong>is also a laboratory work that focuses on <em>In vitro cleavage of target DNA using CRISPR\/Cas9<\/em>. It involves a theoretical-practical session (1h) and a lab work session (3h). The objective of the hands-on activity is to perform a CRISPR\/Cas9 reaction, run the DNA <em>in vitro<\/em> digestion in a gel, interpret the results and discuss. The product of the DNA digestion (from CRISPR\/Cas9 reaction) will be visualized on an agarose gel, a standard lab procedure to separate DNA by size; shorter DNA fragments migrate through the gel more quickly than longer ones. The general learning outcome of these two steps (9 and 10): students should be able to apply hands-on activity to set up an in vitro CRISPR\/Cas9 cut of a target DNA fragment.&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>11<\/strong><strong><sup>th<\/sup><\/strong><strong> step \u2013 <\/strong>can be dedicated to a new phase of the project development, in which students are involved in designing and prototyping a solution for a problem. Through teamwork and collaboration, students focus on their projects on CRISPR (2h) trying to define what methods can they use and what lab work is necessary to make it a reality.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">This phase is dedicated to the scientific exploration of the creative realm. Students generate ideas, brainstorm, and discuss in this ideation phase. This can be followed by a hybrid mentoring session (2h) where students engage and discuss with researchers how to make their projects possible while gaining awareness of the limitations of the scientific process. In terms of Learning Outcomes, students will learn about scientific issues and will develop creative thinking.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>12<\/strong><strong><sup>th<\/sup><\/strong><strong> step \u2013 <\/strong>should be a practical session where students can work autonomously in adjusting their projects, and prepare the materials for the projects\u2019 presentations.&nbsp;&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>13<sup>th<\/sup> step \u2013<\/strong> should be dedicated to the presentation of projects and to a discussion on the CRISPR powers and limitations from a STEAM perspective, involving students in a multidisciplinary reflection and discussion.&nbsp;&nbsp;<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-stackable-spacer stk-block-spacer stk--no-padding stk-block stk-22e659a\" data-block-id=\"22e659a\"><style>.stk-22e659a{height:115px !important}<\/style><\/div>\n","protected":false},"excerpt":{"rendered":"<p>What\u2019s CRISP-R? (general explanation and debate)&nbsp; | 2h | THEORY Arts assignments or creative prompts to promote creativity | 2h | PRACTICAL Exploring the art\/science relationship and the concept of bio-art | 2h | THEORY Basic concepts of molecular biology: DNA, protein, genes, RNA | 2h | THEORY Introduction to gene edition &#8211; hands-on &amp; 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